Priprema biblioteke za “Next generation” sekvenciranje

Sample Prep

NEBNext® reagensi su niz visoko čistih reagensa koji olakšavaju pripremu uzorka DNA ili RNA za "next generation sequencing"te za ciljno obogaćivanje DNA. Dostupni za širok raspon tipova uzoraka, NEBNext proizvodi dolaze u user-friendly formatu, uključujući setove i module. Adapteri i primeri su dostupni odvojeno, za maksimiziranim fleksibilnost. Ovi robusni reagensi prolaze stroge kontrole kvalitete i funkcionalnu provjeru valjanosti, osiguravajući maksimaln prinos, praktičnost i vrijednost za novac.

NEBNext reagensi su dostupni za pripremu uzorka na Illumina®, Ion Torrent ™ i 454 ™ platformama.


POPIS PROIZVODA:

NEBNext Direct Cancer HotSpot Panel  NOVO!!

NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

NEBNext® Ultra™ II Ligation Module 

NEBNext® Ultra™ II Q5® Master Mix

NEBNext® Ultra™ II End Repair/dA-Tailing Module


DNA Master Mix Sets

NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

NEBNext® DNA Library Prep Master Mix Set for 454™

NEBNext® DNA Library Prep Master Mix Set for Illumina®

NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

NEBNext® Fast DNA Library Prep Set for Ion Torrent™

NEBNext® FFPE DNA Repair Mix

NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

NEBNext® Library Quant Kit for Illumina®


DNA Reagent Sets

NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

NEBNext® DNA Library Prep Reagent Set for Illumina®

NEBNext® FFPE DNA Repair Mix

NEBNext® Library Quant Kit for Illumina®


DNA Modules

NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

NEBNext® dA-Tailing Module

NEBNext® dA-Tailing Reaction Buffer

NEBNext® End Repair Module

NEBNext® End Repair Reaction Buffer

NEBNext® FFPE DNA Repair Mix

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

NEBNext® Quick Ligation Module

NEBNext® Quick Ligation Reaction Buffer

NEBNext® Singleplex Oligos for Illumina®

NEBNext® Ultra™ End Repair/dA-Tailing Module

NEBNext® Ultra™ Ligation Module

NEBNext® Ultra™ II Ligation Module 

NEBNext® Ultra™ II End Repair/dA-Tailing Module

NEBNext® Library Quant Kit for Illumina®


DNA Enzymes

NEBNext® High-Fidelity 2X PCR Master Mix

NEBNext® dsDNA Fragmentase® Reaction Buffer

NEBNext® dsDNA Fragmentase®

NEBNext® dsDNA Fragmentase® Reaction Buffer v2

NEBNext® FFPE DNA Repair Mix

NEBNext® Q5® Hot Start HiFi PCR Master Mix


Microbiome Sequencing

NEBNext® Microbiome DNA Enrichment Kit

NEBNext® Library Quant Kit for Illumina®


RNA Master Mix Sets

NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

NEBNext® mRNA Library Prep Master Mix Set for Illumina®

NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

NEBNext® Library Quant Kit for Illumina®

 


RNA Reagent Sets

NEBNext® mRNA Library Prep Reagent Set for Illumina®

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

NEBNext® Library Quant Kit for Illumina®


RNA Modules

NEBNext® Magnesium RNA Fragmentation Module

NEBNext® mRNA Second Strand Synthesis Module

NEBNext® Poly(A) mRNA Magnetic Isolation Module

NEBNext® Q5® Hot Start HiFi PCR Master Mix

NEBNext® RNA First Strand Synthesis Module

NEBNext® RNase III RNA Fragmentation Module

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

NEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer

NEBNext® Ultra™ Directional RNA Second Strand Synthesis Module

NEBNext® Library Quant Kit for Illumina®


Library Preparation for Next Generation Sequencing Buffers & Diluents

NEBNext® dA-Tailing Reaction Buffer

NEBNext® dsDNA Fragmentase® Reaction Buffer

NEBNext® dsDNA Fragmentase® Reaction Buffer v2

NEBNext® End Repair Reaction Buffer

NEBNext® Quick Ligation Reaction Buffer

NEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer